CM 3321 - Biochemistry Lab (1) SP

The biochemistry laboratory course includes the determination of protein concentration, enzyme kinetics, SDS-polyacrylamide gel electrophoresis, isoelectric focusing and protein isolation by high speed centrifugation and various chromatographic techniques. Additionally, the manipulation of DNA including the transformation of E.coli with a plasmid containing a foreign gene, expression of that gene, isolation of the plasmid from the bacteria and restriction analysis of the plasmid on agarose gels.